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排序方式: 共有236条查询结果,搜索用时 46 毫秒
51.
Fumiko TAGUCHI Rui TANAKA Sayuri KINOSHITA Yuki ICHINOSE Yoshiyuki IMURA Salamah ANDI Kazuhiro TOYODA Tomonori SHIRAISHI Tetsuji YAMADA 《Journal of General Plant Pathology》2001,67(2):116-123
To elucidate the role of harpins produced by Pseudomonas syringae, the corresponding hrpZ gene was isolated from P. s. pv. tabaci. The sequence information revealed that this gene carries a serious mutation with 326 bp lacking in the central region and
potentially encodes only 140 N-terminal amino acids because of a frame shift. The investigation of biological properties using
recombinant harpin indicated harpinpsta was incapable of inducing HR in both host and nonhost plants. Based on an immunoblot analysis to detect harpin from P. s. pathovars in hrp-inducing medium, the truncated harpinpsta was neither expressed nor secreted into the culture medium. These results suggest that harpin is not the sole determinant
of the host-parasite specificity in P. s. pv. tabaci.
Received 10 August 2000/ Accepted in revised form 21 December 2000 相似文献
52.
Masaki Nishio Kaneaki Edo Ryosuke Kawakami Tomonori Kawamoto Yuji Yamazaki 《Ecology of Freshwater Fish》2020,29(2):197-209
Adaptations that maximise food intake are dependent on food and/or habitat types. However, there have been few studies on the ability of floodplain primary freshwater fish to rapidly change their morphology, especially the internal one, to maximise utilisation efficiency of food resources in response to environmental fluctuations during their life history. We investigated morphological changes of an Acheilognathinae species (Acheilognathus longipinnis) that inhabits creeks in Japan to determine whether body shape variation correlated with environmental characteristics, including water depth, current velocity, vegetation cover and food availability. When shifting from floodwater-level to low-water-level season, the current velocity of the study area increased as the water level decreased, causing a significant increase in periphyton and a significant decline in zooplankton and phytoplankton. Concurrently, during the short period when A. longipinnis grow from the juvenile to the premature stage, changes in internal morphology, that is intestinal elongation, and changes in external morphology such as an increase of body depth were observed. In contrast, intestinal length significantly decreased in the adult stage. Our findings suggest that morphological changes between floodwater-level and low-water-level seasons associated with juvenile to premature development are an adaptation to changes in food availability due to environmental fluctuations. Additionally, the shortening of the intestine from the premature to the adult stage may be an adaptive strategy for reproduction. 相似文献
53.
Yosuke Iimura Madoka Yoshizumi Tomonori Sonoki Mikiko Uesugi Kenji Tatsumi Ken-ichi Horiuchi Shinya Kajita Yoshihiro Katayama 《Journal of Wood Science》2007,53(6):541-544
To assess the possible utility of a fungal gene for manganese-dependent peroxidase (MnP) produced by a transgenic plant in
phytoremediation, we transformed hybrid aspen with a chimeric gene for MnP. Our gene construct allowed expression of the gene
for MnP in plants and relatively high MnP activity was detected in the hydroponic medium in which roots of plants that expressed
the transgene had been cultured. Some of our transgenic plants were able to remove bisphenol A from the medium more efficiently
than wild-type plants. Our results demonstrate that, without any modification of the coding sequence, a chimeric gene for
fungal MnP can be expressed in a woody plant, with secretion of active MnP from roots into the rhizosphere. Our strategy suggests
new options using woody plants for phytoremediation. 相似文献
54.
Takasu M Shirota K Uchida N Iguchi T Nishii N Ohba Y Maeda S Miyazawa K Murase T Kitagawa H 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2006,68(5):519-521
We encountered an extremely rare tumor, a pericardial mesothelioma, in a neonatal calf. The patient calf showed severe abdominal distention, and died immediately after birth. The thoracic cavity was contained a huge heart with a large amount of pericardial fluid. A number of granular and cobblestone-like nodules were dispersed over the epicardium and pericardium. The nodules consisted of papillary proliferations of neoplastic cells, and the neoplasm occasionally showed mesenchymal proliferations. Immunohistochemistry revealed that they had the characteristics of mesothelial cells (cytokeratin-and vimentin-positive), and the neoplasm was diagnosed as mesothelioma. 相似文献
55.
Akinori Kiba Takako Ohgawara Kazuhiro Toyoda Miho Inoue-Ozaki Tadahiro Takeda Uppalapati Srinivasa Rao Toshiaki Kato Yuki Ichinose Tomonori Shiraishi 《Journal of General Plant Pathology》2006,72(4):228-237
In the plant cell wall of Pisum sativum seedlings, we found an NTPase (E.C. 3.6.1.5.) with ATP-hydrolyzing activity that was regulated by an elicitor and suppressors
of defense from pea pathogen Mycosphaerella pinodes. The ATPase-rich fraction was purified from pea cell walls by NaCl solubilization, ammonium sulfate precipitation, and chromatography
with an ATP-conjugated agarose column and an anion-exchange column. The specific activity of the final ATPase-rich fraction
increased 600-fold over that of the initial NaCl-solubilized fraction. The purified ATPase-rich fraction also had peroxidase
activity and generated superoxide, both of which were regulated by the M. pinodes elicitor and suppressor (supprescins). Active staining and Western blot analysis also showed that the ATPase was copurified
along with peroxidases. In this fraction, a biotinylated elicitor and the supprescins were bound primarily and specifically
to ca. 55-kDa protein (CWP-55) with an N-terminal amino acid sequence of QEEISSYAVVFDA. The cDNA clone of CWP-55 contained five ACR domains, which are conserved in
the apyrases (NTPases), and the protein is identical to a pea NTPase cDNA (GenBank accession AB071369). Based on these results, we discuss a role for the plant cell wall in recognizing exogenous
signal molecules. 相似文献
56.
Yamashita K Fujiwara C Azuma R Sawazaki T Nakao Y Hasegawa A 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2002,64(8):673-676
Housedust mites, Dermatophagoides pteronyssinus (D. pteronyssinus) and Dermatophagoides farinae (D. farinae), are the important causative agents of allergic diseases in human and animals. By using 165 dogs suffering from atopic dermatitis (AD), serum levels of immunogloblin E (IgE) antibody against 25 kinds of allergen including housedust mites were determined. Housedust mites were the most frequent allergen against which 90 of the 165 allergic dogs (54.5%) by IMMUNODOT assay. With the further analysis of immunoblotting assay in the 90 dogs sensitized with housedust mites, antigenic proteins of housedust mites recognized by IgE antibodies were with the apparent molecular masses of 15, 76, 90, 98, and 170-kD. Among them, the 15-kD protein that might be identical to Group 2 antigens (Der f2, Der p2) was prominently observed (52/90). This study indicates that about a half of dogs with AD were sensitized to housedust mites, suggesting that Group 2 antigens of housedust mites may be a major allergen in canine AD. 相似文献
57.
Hiroyuki Takahara Kazuhiro Toyoda Gento Tsuji Yasuyuki Kubo Yoshishige Inagaki Yuki Ichinose Tomonori Shiraishi 《Journal of General Plant Pathology》2005,71(3):190-195
Mycosphaerella blight, caused by Mycosphaerella pinodes, is one of the major diseases of cultivated pea (Pisum sativum L.). To isolate the genes that are up- and down-regulated during spore germination, suppression subtraction hybridization (SSH) was performed between ungerminated and germinated spores. The 232 and 128 clones from forward and reverse libraries, respectively, were collected, sequenced, and analyzed with a BLASTX homology search. About 95% of the 32 selected clones were expressed during spore germination on a paper sheet and during infection of pea leaves. We discuss the applicability of the SSH libraries for analyzing M. pinodes genes involved in the early stage of infection. 相似文献
58.
A common approach to land use change analyses in multidisciplinary landscape-level studies is to delineate discrete forest
and non-forest or urban and non-urban land use categories to serve as inputs into sets of integrated sub-models describing
socioeconomic and ecological processes. Such discrete land use categories, however, may be inappropriate when the socioeconomic
and ecological processes under study are sensitive to a range of human habitation. In this paper, we characterize the spatial
dynamic distribution of humans throughout the forest landscape of western Oregon (USA). We develop an empirical model describing
the spatial distribution and rate of change in historic building densities as a function of a gravity index of development
pressure, existing building densities, slope, elevation, and existing land use zoning. We use the empirical model to project
changes in building densities that are applied to a 1995 base map of building density to describe future spatial distributions
of buildings over time. The projected building density maps serve as inputs into a multidisciplinary landscape-level analysis
of socioeconomic and ecological processes in Oregon's Coast Range Mountains.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
59.
Ohba Y Iguchi T Hirose Y Takasu M Nishii N Maeda S Kitagawa H 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2008,70(8):829-831
A 24-day-old female Holstein calf had a soft, painless fluctuating swelling on the median plane in the frontal region, but did not show any clinical symptoms including neurological signs. Computer tomography (CT) distinctly showed the cyst filled with fluid and part of the encephalon. Hence, this swelling was diagnosed as meningoencephalocele, but not meningocele. The meningoencephalocele was successfully repaired surgically. Meningoencephalocele can thus be easily recognised by CT in a calf. 相似文献
60.